Sero-prevalence of bovine brucellosis in Kuku Dairy Scheme, Khartoum North, Sudan


By T. E. E. Angara, A. A. Ismail, H. Agab, N. S. Saeed.


A sero-prevalence study was conducted in Kuku Dairy Scheme, Khartoum North, Sudan. The scheme was proved to be endemic with bovine brucellosis. Cross-reaction with other bacteria and the possibility of false positive reactor animals due to vaccination had justified the use of competitive ELISA test for serum detection as a confirmatory test. The number of cattle examined, throughout the study, was 574 out of 845 cows kept in Kuku Dairy Scheme. All the obtained sera were screened using Rose Bengal Plate Test (RBPT). Twenty eight out of the thirty herds of the sample had at least one positive reactor, resulting in 93.3% herd prevalence rate. All sera positive to Rose Bengal Plate Test (n = 178) were subjected to further confirmatory test using Competitive Enzyme-Linked Immunosorbent Assay (c-ELISA). 143 cows (80.3%) were confirmed positive by c-ELISA. Out of 28 positive herds, 27 (96%) had at least one positive reactor. According to the confirmatory test, the herd prevalence rate was 90%, individual animal prevalence rate was 24.9% and average within herd prevalence rate was 24.5% (±15.7, CI 4.088 at 95%). The number of seropositive aborter was found to be 17 cows out of 143 (12% ). It is concluded that bovine brucellosis was highly prevalent in Kuku Dairy Scheme. This fact justifies immediate adoption of an effective control policy for this zoonotic disease.


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